| Characterization of Invading Glioma Cells Using Molecular Analysis of Leading-Edge Tissue. |
| Cheol Soo Kim, Shin Jung, Tae Young Jung, Woo Youl Jang, Heung Suk Sun, Hyang Hwa Ryu |
1Department of Neurosurgery, Chonnam National University Research Institute of Medical Sciences, the Brain Korea 21 Project, Chonnam National University Hwasun Hospital & Medical School, Gwangju, Korea. sjung@chonnam.ac.kr
2Brain Tumor Research Laboratory, Chonnam National University Research Institute of Medical Sciences, the Brain Korea 21 Project, Chonnam National University Hwasun Hospital & Medical School, Gwangju, Korea.
3Department of Clinical Laboratory Science, Seonam University, Gwangju, Korea. |
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| ABSTRACT |
OBJECTIVE
We have introduced a method of characterization of invading glioma cells by using molecular analysis of marginal invading tumor cells and molecular profiles of glioma tumor margin.
METHODS
Each of tumor core and marginal tissues was obtained in 22 glioma patients. Tumor core cells and marginal cells from each glial tumor were collected by laser capture microdissection or intraoperative microdissection under the operating microscope. Expression of MMP-2, MMP-9, CD44 and RHAMM mRNA by invading glioma cells compared with tumor core was confirmed by realtime-PCR of twenty-four glioma specimens. Clinical data also were reviewed for invasion and recurrence pattern of the gliomas radiologically and invasive rim pattern microscopically.
RESULTS
Overall results of the molecular analysis showed that relative overexpression of MMP-2, MMP-9 and RHAMM were noted at the invasive edge of human glioma specimens comparing to the tumor core but CD44 was highly expressed in the tumor core comparing to the margin. High marginal expression of MMP-2 and MMP-9 were noted in poorly ill-defined margin on the pathological finding. High marginal expression of CD44 and MMP-2 were demonstrated in the midline cross group on the radiological review, and that of RHAMM and MMP-2 were showed in the aggressive recurrence group. High expression of MMP-2 seems to be involved in the various invasion-related phenomenons.
CONCLUSION
Up-regulation of MMP-2, MMP-9, CD44 and RHAMM was noted in invasive edge of gliomas according to the various clinical situations. |
| Key Words:
CD44; Glioma; Laser capture nicrodissection; MMP-2; MMP-9; RHAMM |
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