| Home | E-Submission | Sitemap | Editorial Office |  
top_img
Journal of Korean Neurosurgical Society 1986;15(3): 511-520.
The Effect of Indomethacin on Na-K-ATPase and K-pNPPase Activity of Rat Brain.
Ki Uk Kim, Young Tae Kim, Sang Yong Kim, Young Woo Lee
1Department of Neurosurgery, Yonsei University, College of Medicine, Seoul, Korea.
2Department of Neurosurgery, School of Medicine, Pusan National University, Korea.
ABSTRACT
The effect of indomethacin on Na-K-ATPase and K-pNPPase activity was studied with rat brain homogenate. The results were as follows : 1) Indomethacin inhibited both Na-K-ATPase and K-pNPPase in a dose-related pattern. 2) Inhibition mode of indomethacin for K+ in both Na-K-ATPase and K-pNPPase was competitive type. 3) Indomethacin showed stimulative effect at lower sodium concentration below 10mM, and showed inhibitory effect at higher sodium concentration on Na-K-ATPase and K-pNPPase activity, and the inhibitory effect was slightly increased with increasing concentration of sodium. 4) The inhibitory effect of indomethacin on Na-K-ATPase activity was increased with increasing ATP concentration, but was not affected by Mg++ concentration. These results indicate that indomethacin inhibits Na-K-ATPase activity by inhibiting K+- dependent dephosphorylation steps.
Key Words: Indomethacin; Na-K-ATPase; K-pNPPase; Lineweaver-Burk plot; K+- dependent dephosphorylation
Editorial Office
1F, 18, Heolleung-ro 569-gil, Gangnam-gu, Seoul, Republic of Korea
TEL: +82-2-525-7552   FAX: +82-2-525-7554   E-mail: kns61@neurosurgery.or.kr
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © Korean Neurosurgical Society.                 Developed in M2PI
Close layer