Loss of Heterozygosity of Chromosome 17p in Brain Tumors.

Baek, Sun Ha; Kim, Eun Sang; Kim, Dong Gyu; Jung, Hee Won; Kim, Hyun Jib; Cho, Byung Kyu; Han, Dae Hee; Lee, Seong Hun; Kim, Jong Hyun

Original Article

Journal of Korean Neurosurgical Society 1992;21(1): 23-29.

Sun Ha Baek, Eun Sang Kim, Dong Gyu Kim, Hee Won Jung, Hyun Jib Kim, Byung Kyu Cho, Dae Hee Han, Seong Hun Lee, Jong Hyun Kim

¹Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea.
²Department of Neurosurgery, Korea Cancer Center Hospital, Seoul, Korea.

ABSTRACT

With the methods of restriction fragment length polymorphisms(RFLPs) and southern blot analysis, gene deletion of chromosome 17p in 16 cases of brain tumors, was investigated. There were 4 cases of glioblastoma multiforme, 1 case of anaplastic astrocytoma, 4 cases of low grade astrocytoma, 3 cases of oligodendroglioma, and 4 cases of meningioma. Among restriction fragment length polymorphism(RFLP) DNA located in chromosome 17p, p144D 6 and p SNZ 22 were imployed as the probes. In eight of 16 cases(50%) constitutional heterozygosity was observe dfor p144 D6 probe on the short arm of chromosome 17, and in nine of 16 cases(56%) for PYNZ 22.1 probe. With both probes constitutional heterozygosity was observed in thirteen of 16 cases(81%). And the loss of constitutional heterozygosity was detected in two of 14 informative cases. Although, with the malignant gliomas, including 4 cases of glioblastoma multiforme and 1 case of anaplastic astrocytoma, two of 4 informative cases showed loss of constitutional heterozygosty, None of 9 informative cases showed loss of heterozygosity with the other brain tumors(low grade astrocytoma, oligodendroglioma, and meningioma).

Key Words: RFLP; Southern blot analysis; Glioblastoma multiforme; Anaplastic astrocytoma; Loss of heterozygosity