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Journal of Korean Neurosurgical Society 1992;21(7): 813-825.
A Study on the Change of Xanthine Oxidase Activities in Experimental Cerebral Infarction.
Byung Kook Min, Kwan Park, Young Baeg Kim, Seung Nam Hwang, Jong Sik Suk, Duck Young Choi, Yong Seok Park, Young Bae Lee
1Department of Neurosurgery, College of Medicine, Chungang University, Seoul, Korea.
2Department of Neurosurgery, College of Medicine, Dongguk University, Kyungju, Korea.
To observe the activity of the xanthine oxidase in cerebral ischemia, an ischemic model was made using a transorbital occlusion of the middle cerebral artery(MCA) in cats. Xanthine oxidase activity was measured by means of high performance liquid chromatography with electrochemical detection of uric acid. The results were as follows: The infarction in the territory of the left middle cerebral artery(MCA) was identified with intracardiac perfusion of a 2% triphenyltetrazolium chloride(TTC) solution after transorbital occusion. 2) There was no significant difference between the control and ischemia groups in the total xanthine oxidase activity. 3) In the control group, 38.2% of the total xanthine oxidase was in the oxygen-dependent superoxide-producing oxidase group(Type 0). It had been increased with time in ischemia, despite the total xanthine oxidase activity remaining the same;particularly at 12 hours(p<0.05) and 24 hours(p<0.01) after occlusion of the left MCA. 4) In the healthy side, the activity of type 0 and its ratio to the total xanthine oxidase activity has not been increased. 5) In the ischemic side, the conversion of type D xanthine oxidase to the type 0 xanthine oxidase was demonstrated and its rate had been increased with the passage of time.
Key Words: Cerebral ischemia; Free radicals; High-performance liquid chromatography; Xanthine oxidase
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