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Journal of Korean Neurosurgical Society 1994;23(12): 1359-1368.
Phorbol Ester Modulates th Action of Acetylcholine in Rabbit Carotid Artery.
Yong Lae Nho, Sang Ho Lee, Young Woo Lee
1Department of Neurosurgery, College of Medicine, Pusan National University, Pusan, Korea.
2Department of Physiology, College of Medicine, Pusan National University, Pusan, Korea.
ABSTRACT
Authors studied the regulatory mechanism of protein kinase C on the action of acetylcholine in rabbit carotid artery. The arterial rings were myographied isometrically in an isolated organ bath. In this study, acetylcholine relaxed phenylephrine-induced contraction of rabbit carotid artery in the presence of endothelium. In the pretreatment of methylene blue or nitro-L-arginine, the action of acetylchioline was reduced. Pretreatment of phorbol 12-myristate 13-acetate(PMA) attenuated the action of acetylcholine, but PMA did not attenuated it in the presence of staurosporine, suggesting that protein kinase C suppressed the action of acetylcholine. The potency of phorbol ester on the action of acetylcholine was PMA>phorbol 12, 13-dibutyrate(PDBu)>phorbol 12,13-diacetate(PDA), but the direct effect of phorbol on the contraction of arterial rings was PDBu>PMA>PDA. This implied that protein kinase C involved in the contraction of smooth muscle and the attenuation of the action of acetylcholine were different. PMA did not affect on A23187- and sodium nitroprusside-induced vasorelaxation. Acetylcholine increased tissue cGMP contents, which was reduced by PMA. These results suggest that in rabbit carotid artery protein kinase C reduce acetylcholine-stimuated endothelium derived relaxing factor(EDRF) release by affecting membrane receptor, and do not affect on the function of EDRF and cGMP production in the smooth muscle.
Key Words: Phorbol ester; Acetylcholine; Protein kinase C; Contraction of smooth muscle; Endothelium derived relaxing factor(EDRF)
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