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Journal of Korean Neurosurgical Society 1995;24(11): 1292-1299. |
| Contractile Effects of Cerebrospinal Fluid from Patients with Brain Lesion on Porcine Isolated Brain Arteries. |
| Tai Ho Kim, Sam Suk Kang, Yung Hong Baik |
1Department of Neurosurgery, Chonnam University Medical School, Kwangju, Korea.
2Department of Pharmacology, Chonnam University Medical School, Kwangju, Korea. |
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| ABSTRACT |
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It is well known that bloody cerebrospinal fluid(BCSF) obtained from patient with subarachnoid hemorrhage will contracts isolated brain arteries, but the effects of non-bloody CSF(non-BCSF), which does not contain blood components, has not been reported yet. In this study, CSFs were obtained from 12 patients hospitalized at the Department of Neurosurgery, Chonnam University Hospital from February to September 1994. Among the CSFs from these 12 patients, 3 cases were bloody and 9 cases were not. It was found that all BCSFs produced marked contractions in ring preparations of porcine isolated basilar and circle of Willis arteries. The non-BCSFs fell into two groups:one which contracted the preparations in a volume-dependent fashion(30-1000 microliter) and the other did not. The purpose of this study was to investigate the mechanism(s) of contractile response to the first group on-BCSF in the porcine isolated brain artery. Concentration-dependent contractions induced by KCl were not affected by the pretreatment with 500 microliter non-BCSF, while contraction induced by 5-hydroxytryptamine(5-HT) and phenylephrine(PE) were significantly potentiated by the same pretreatment of non-BCSF. Although volume-dependent contractions induced by the non-BCSF were not affected by the pretreatment with 10(-6) M phentolamine and 10(-4) M L-NAME, the contractions was significantly attenuated by the presence of 10(-6) M indomethacin and nimodipine. It was also found that the contraction induced by prostaglandin F2alpha(PGF2alpha) was relaxed by nitroprusside and atriopeptin(ANP) in a concentration-dependent manner and such relaxations were not affected by nitroprusside and ANP were not affected in the presence of 500 microliter non-BCSF. Above observations suggest that components, of non-BCSF from patients with brain lesion contains an unknown substance(s) that induces contraction of porcine isolated brain arteries and the above contractile effect involves the extracellular calcium influx and/or cyclooxygenaseprostaglandin system but not the NO-guanylate cyclase system. |
| Key Words:
Non-bloody CSF; Porcine brain artery; Contractile effect |
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