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Journal of Korean Neurosurgical Society 2004;36(4): 317-323.
The Apoptosis and Expression of p53, Bcl-2 in Graded Contusion Injury of Rat Spinal Cord.
Ung Kyu Chang, Woo Jin Choe, Ki Jeong Kim, Chun Kee Chung, Hyun Jib Kim
1Department of Neurosurgery, Seoul National University College of Medicine, Clinical Research Institute, Seoul, Korea.
2Department of Neurosurgery, Korea Cancer Center Hospital, Seoul, Korea. cuk@kcch.re.kr
3Department of Neurosurgery, Inje University College of Medicine, Seoul, Korea.
ABSTRACT
OBJECTIVE
This study is designed to investigate how apoptosis is presented and how the genes of p53 and bcl-2 are expressed depending on graded injury in experimental spinal cord injury. METHODS: Experimental spinal cord injury was made on rats with weight drop method. Two different amounts of impact were applied on rat spinal cord. Rats were categorized into three groups (control; five rats, mild injury; five rats, severe injury; five rats). Fourty eight hours following cord injury, cord specimen was harvested from injury epicenter. TUNEL staining was done for apoptotic detection and immunohistochemical staining for p53 and bcl-2 expression. Positively stained cells were counted and mean values were compared among three groups. RESULTS: TUNEL positive cells increased depending on injury severity(p=0.027). The p53 positive cells increased in both injury groups compared to control group(p=0.001). Bcl-2 positive cells decreased as injury amount increased(p=0.002). The p53 expression increased in proportion to TUNEL staining in correlation curve in white matter(correlation coefficient, 0.387). The bcl-2 expression was inversely proportional to TUNEL staining and steeper decrease was found in gray matter than in white matter (correlation coefficient, -0.875). CONCLUSION: Apoptosis increases as the injury grading elevated within 20gm-cm of impact. The p53 seems to promote apoptosis in white matter, but do not show proportional relationship with injury amount. Bcl-2 appeared to be protective to cell death due to apoptosis.
Key Words: Spinal cord injury; Apoptosis; p53; Bcl-2
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