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Journal of Korean Neurosurgical Society 2005;37(1): 48-53.
The Combined Effect of Gamma Knife Irradiation and p53 Gene Transfection in Human Malignant Glioma Cell Lines.
Jeong Eun Kim, Sun Ha Paek, Dong Gyu Kim, Hyun Tai Chung, Young Yim Kim, Hee Won Jung
1Department of Neurosurgery, Seoul National University College of Medicine, Seoul, Korea. hwnjung@snu.ac.kr
2Clinical Research Institute, Seoul National University College of Medicine, Seoul, Korea.
The purpose of this study is to elucidate in vitro responses to combined gamma knife irradiation and p53 gene transfection on human malignant glioma cell lines.
Two malignant human glioma cell lines, U87MG (p53-wild type) and U373MG (p53-mutant) were transfected with an adenoviral vector containing p53 (MOI of 50) before and after applying 20Gy of gamma irradiation. Various assessments were performed, including, cell viability by MTT assay; apoptosis by annexin assay; and cell cycle by flow cytometry, for the seven groups: mock, p53 only, gamma knife (GK) only, GK after LacZ, LacZ after GK, GK after p53, p53 after GK. RESULTS: Cell survival decreased especially, in the subgroup transfected with p53 after gamma irradiation. Apoptosis tended to increase in p53 transfected U373 MG after gamma irradiation (apoptotic rate, 38.9%). The G2-M phase cell cycle arrest markedly increased by transfecting with p53, 48 hours after gamma knife irradiation in U373 MG (G2-M phase, 90.8%). CONCLUSION: These results suggest that the in vitro effects of combined gamma knife irradiation and p53 gene transfection is an augmentation of apoptosis and G2-M phase cell cycle arrest, which are more exaggerated in U373 MG with p53 transfection after gamma knife irradiation.
Key Words: Adenovirus; Gamma knife; Gene therapy; Malignant glioma; p53; Radiation
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