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Journal of Korean Neurosurgical Society > Volume 46(4); 2009 > Article
Journal of Korean Neurosurgical Society 2009;46(4): 397-402.
doi: https://doi.org/10.3340/jkns.2009.46.4.397
Evaluation of Spinal Fusion Using Bone Marrow Derived Mesenchymal Stem Cells with or without Fibroblast Growth Factor-4.
Hyun Sung Seo, Jong Kwon Jung, Mi Hyun Lim, Dong Keun Hyun, Nam Sik Oh, Seung Hwan Yoon
1Department of Neurosurgery, School of Medicine, Inha University, Incheon, Korea. nsyoon@gmail.com
2Department of Physiology, School of Medicine, Inha University, Incheon, Korea.
3Department of Anesthesiology, School of Medicine, Inha University, Incheon, Korea.
4Department of Dental, School of Medicine, Inha University, Incheon, Korea.
5Cell Therapy Research Center, School of Medicine, Inha University, Incheon, Korea.
ABSTRACT
OBJECTIVE:
In this study, the authors assessed the ability of rat bone marrow derived mesenchymal stem cells (BMDMSCs), in the presence of a growth factor, fibroblast growth factor-4 (FGF-4) and hydroxyapatite, to act as a scaffold for posterolateral spinal fusion in a rat model.
METHODS:
Using a rat posterolateral spine fusion model, the experimental study comprised 3 groups. Group 1 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite only. Group 2 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing 1 x 10(6)/ 60 microliter rat of BMDMSCs. Group 3 was composed of 6 animals that were implanted with 0.08 gram hydroxyapatite containing 1 x 10(6)/ 60 microliter of rat BMDMSCs and FGF-4 1 microgram to induce the bony differentiation of the BMDMSCs. Rats were assessed using radiographs obtained at 4, 6, and 8 weeks postoperatively. After sacrifice, spines were explanted and assessed by manual palpation, high-resolution microcomputerized tomography, and histological analysis.
RESULTS:
Radiographic, high-resolution microcomputerized tomographic, and manual palpation revealed spinal fusion in five rats (83%) in Group 2 at 8 weeks. However, in Group 1, three (60%) rats developed fusion at L4-L5 by radiography and two (40%) by manual palpation in radiographic examination. In addition, in Group 3, bone fusion was observed in only 50% of rats by manual palpation and radiographic examination at this time.
CONCLUSION:
The present study demonstrates that 0.08 gram of hydroxyapatite with 1 x 10(6)/ 60 microliter rat of BMDMSCs induced bone fusion. FGF-4, added to differentiate primitive 1 x 10(6)/ 60 microliter rat of BMDMSCs did not induce fusion. Based on histologic data, FGF-4 appears to induce fibrotic change rather than differentiation to bone by 1 x 10(6)/ 60 microliter rat of BMDMSCs.
Key Words: Bone marrow derived mesenchymal stem cell; Fibroblast growth factor-4; Hydroxyapatite; Spinal fusion
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