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Journal of Korean Neurosurgical Society 2010;47(3): 203-209.
doi: https://doi.org/10.3340/jkns.2010.47.3.203
Blood-Brain Barrier Experiments with Clinical Magnetic Resonance Imaging and an Immunohistochemical Study.
Jun Woo Park, Hak Jin Kim, Geun Sung Song, Hyung Soo Han
1Department of Neurosurgery, College of Medicine & Medical Research Institute, Pusan National University, Yangsan, Korea. gnsong@pusan.ac.kr
2Department of Radiology, College of Medicine & Medical Research Institute, Pusan National University, Yangsan, Korea.
3Department of Physiology, Kyungpook National University School of Medicine, Daegu, Korea.
The purpose of study was to evaluate the feasibility of brain magnetic resonance (MR) images of the rat obtained using a 1.5T MR machine in several blood-brain barrier (BBB) experiments.
Male Sprague-Dawley rats were used. MR images were obtained using a clinical 1.5T MR machine. A microcatheter was introduced via the femoral artery to the carotid artery. Normal saline (group 1, n = 4), clotted autologous blood (group 2, n = 4), triolein emulsion (group 3, n = 4), and oleic acid emulsion (group 4, n = 4) were infused into the carotid artery through a microcatheter. Conventional and diffusion-weighted images, the apparent coefficient map, perfusion-weighted images, and contrast-enhanced MR images were obtained. Brain tissue was obtained and triphenyltetrazolium chloride (TTC) staining was performed in group 2. Fluorescein isothiocyanate (FITC)-labeled dextran images and endothelial barrier antigen (EBA) studies were performed in group 4.
The MR images in group 1 were of good quality. The MR images in group 2 revealed typical findings of acute cerebral infarction. Perfusion defects were noted on the perfusion-weighted images. The MR images in group 3 showed vasogenic edema and contrast enhancement, representing vascular damage. The rats in group 4 had vasogenic edema on the MR images and leakage of dextran on the FITC-labeled dextran image, representing increased vascular permeability. The immune reaction was decreased on the EBA study.
Clinical 1.5T MR images using a rat depicted many informative results in the present study. These results can be used in further researches of the BBB using combined clinical MR machines and immunohistochemical examinations.
Key Words: Animal; Blood-brain barrier; Magnetic resonance image; Immunohistochemical study; Vasogenic edema; Vascular permeability
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